Pharmacodynamics of frigid zone plant <i>Taxus cuspidata S. et Z.</i> against skin melanin deposition, oxidation, inflammation and allergy

Abstract Background Taxus cuspidata S. et Z. is a precious species of frigid zone plant belonging to the Taxaceae family, which possesses anticancer, anti-inflammatory, hypoglycemic, and antibacterial pharmacological properties. While taxane extracted from chinensis has been reported elicit antioxidant activities, whether skin-protective actions against injuries remained unknown. This study aims explore effects three extracts on skin melanin deposition, oxidation, inflammation, allergy so as provide new ideas for prevention treatment various diseases related damage. Methods Skin deposition was evaluated by measuring content in guinea pigs alkali lysis method. Antioxidant capacity superoxide dismutase (SOD) concentration glutathione (GSH) tissue homogenates Kunming mice SOD assay kit micro reduced GSH kit. The quantitative real-time polymerase chain reaction (qRT-PCR) western blotting were used examine levels both recombinant peroxidase 4 (GPX4). inflammation xylene-induced ear swelling test egg-white-induced paw mice. In mouse model induced 4-aminopyridine (4-AP), determined licking body counts histamine concentrations using enzyme-linked immunosorbent (ELISA) kits. Two proinflammatory factors tumor necrosis factor (TNF)-α interleukin (IL)-1β measured qRT-PCR. Hematoxylin eosin (HE) staining conducted assess degree lesion. Results All including essential oil, extract compound deposits back relative non-treated control animals, oil produced greatest effect. contrast, elevated tissues, highest increase seen with oil. Three extracts, especially effectively reduce rate swelling. number licks, TNF-α IL-1β, alleviated Consistently, yielded magnitude decreases. Conclusion all possessed anti-skin properties, superior effects.